Autophagy has been linked to longevity in many species. but the underlying mechanisms are unclear. Using a GFP-tagged and a new tandem-tagged Atg8/LGG-1 reporter. we quantified autophagic vesicles and performed autophagic flux assays in multiple tissues of wild-type Caenorhabditis elegans and long-lived daf-2/insulin/IGF-1 and glp-1/Notch mutants throughout adulthood. https://www.spidertattooz.com/Messerschmitt-Me262A-1A-Fighter-Aircraft-LT-Walter-Hagenah-III-Jagdeschwader-7-Larz-Airfield-Germany-1945-German-Luft/